Researchers Pinpoint How Copper Folds Protein into Precursors of Parkinson’s Plaques
Calculations on the Jaguar supercomputer reveal metal binding likely to misfold alpha-synuclein
Aided by the Jaguar supercomputer at Oak Ridge National Laboratory (ORNL), researchers at North Carolina State University have figured out how copper induces misfolding in the protein associated with Parkinson’s disease, leading to creation of the fibrillar plaques which characterize the disease. This finding has implications for both the study of Parkinson’s progression, as well as for future treatments.
The protein in question, alpha-synuclein, is the major component of fibrillar plaques found in Parkinson’s patients. Researchers had already discovered that certain metals, including copper, could increase the rate of misfolding by binding with the protein, but were unsure of the mechanism by which this binding took place.
“We knew that the copper was interacting with a certain section of the protein, but we didn’t have a model for what was happening on the atomic level,” says Frisco Rose, Ph.D. candidate in physics and lead author of the paper describing the research. “Think of a huge swing set, with kids all swinging and holding hands—that’s the protein. Copper is a kid who wants a swing. There are a number of ways that copper could grab a swing, or bind to the protein, and each of those ways would affect all of the other kids on the swing set differently. We wanted to find the specific binding process that leads to misfolding.”
Rose and North Carolina State colleagues Miroslav Hodak, research assistant professor of physics, and Jerzy Bernholc, Drexel Professor of Physics and Director of the Center for High Performance Simulation, developed a series of computer simulations designed to ferret out the most likely binding scenario.
According to Hodak, “We simulated the interactions of hundreds of thousands of atoms, which required multiple hundred thousand CPU-hour runs to study the onset of misfolding and the dynamics of the partially misfolded structures.”
The number of calculations was so large that Hodak and Bernholc had to devise a new method to make it possible for a computer to process them. Only supercomputers like Jaguar, ORNL’s most powerful supercomputer—the most powerful in the United States, in fact—were up to the task. But the simulations finally revealed the binding configuration most likely to result in misfolding.
Their results appear in the June 14 edition of Scientific Reports, an online journal of the Nature Publishing Group.
The researchers hope that their finding will advance our understanding of Parkinson’s, one of the most common—and devastating—neurological diseases. “Understanding the molecular mechanism of Parkinson’s disease should help researchers in developing drugs that treat the disease rather than merely alleviate symptoms,” Bernholc says.
The Department of Energy and the National Science Foundation funded the research. The Innovative and Novel Computational Impact on Theory and Experiment, or INCITE, program, which is jointly managed by the Argonne and Oak Ridge Leadership Computing Facilities, provided the supercomputing time allocation on Jaguar.—by Tracey Peake
Tracey Peake is a public communication specialist at North Carolina State University.